Studies on the application of thin layer isoelectric focusing to food analysis II. Identification of fish species by numerical value of isoelectric focusing pattern.

Identification of Fish Species by Thin-layer Polyacrylamide Gel Isoelectric Focusing

Conventional electrophoretic techniques for the identification offish species are limited in the resolution and reproducibility needed for the reliable identification of fish species. This paper describes the potential of a high resolution protein separation technique, thin-layer polyacrylamide gel isoelectric focusing (IEFl, as a new means of identifying fish species. Sarcoplasmic protein patt...

Isoelectric focusing.

Analysis of rat serum apolipoproteins by isoelectric focusing. II. Studies on the low molecular weight subunits.

The low molecular weight proteins of rat apo HDL and apo VLDL have been isolated and analyzed by the technique of isoelectric focusing. Sephadex fractions from apo HDL (HS-3) and apo VLDL (VS-3) that contain these proteins reveal three major bands with apparent isoelectric points of pH 4.50, 4.67, and 4.74, as well as three minor bands at pH 4.43, 4.57, and 4.61. In addition, apo HDL has a majo...

Analysis of urinary proteins in urolithiasis by isoelectric focusing using ultra-thin-layer gel.

greater in the mutant ( P < 0.00 1 ) and remained constant with age. The heart was not affected. These results indicate that the myotonic mutation has a signficant effect on the total glucose-6-phosphatase activities of skeletal muscle and kidney, but whereas the former is raised the latter is lowered. There is a small effect on the liver and no effect on the heart. Myotonic dystrophy is known ...

Capillary Isoelectric Focusing

Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...